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stui neb cat  (New England Biolabs)


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    New England Biolabs stui neb cat
    Stui Neb Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stui+neb+cat/StuI/pm41819105-327-137-138
    Average 96 stars, based on 646 article reviews
    stui neb cat - by Bioz Stars, 2026-09
    96/100 stars

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    Recombinant:

    Article Title: Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ACTN2 antibody (alpha-actinin), mouse monoclonal, clone EA-53 Sigma-Aldrich Cat#A7811; RRID: AB_476766 Anti-ATP5B antibody, rabbit polyclonal Sigma-Aldrich Cat#HPA001528; RRID: AB_1078242 NDUFA9 antibody, mouse monoclonal IgG, clone 20C11B11B11 Invitrogen Cat#459100; RRID: AB_10376187 Anti-SDHB, mouse monoclonal IgG2a, clone 21A11EA7 Abcam Cat#ab14714; RRID: AB_301432 Anti-UQCRSF1/RISP antibody, mouse monoclonal IgG2b, clone 5A5 Abcam Cat#ab14746; RRID: AB_301445 Doublecortin antibody (DCX), rabbit Cell Signaling Technology Cat#4604; RRID: AB_561007 Chemicals, peptides, and recombinant proteins StemMACSiPS-Brew XF, human Miltenyi Biotec Cat#130-104-368 mTeSRPlus STEMCELL Technologies Cat#100-0276 KnockOut-DMEM Gibco Cat#10829-018 KnockOutserum replacement (SR) Gibco Cat#10828-028 DMEM/F/12 Gibco Cat#31330038 DMEM glucose-free Gibco Cat#11966025 Glasgow-MEM Gibco Cat#11710035 Neurobasal A-Medium glucose-free Gibco Cat#A2477501 Neurobasal Gibco Cat#21103-049 Non-essential amino acids (MEM-NEAA) 100X Gibco Cat#11140-050 Sodium pyruvate Gibco Cat#11360070 B-27 with Vitamin A (50×) Gibco Cat#17504044 B-27 without Vitamin A (50×) Gibco Cat#12587010 N2 Supplement (100×) Gibco Cat#17502-048 DPBS, no calcium, no magnesium Gibco Cat#14190144 Accutase Thermo Fisher Scientific; Sigma-Aldrich Cat#A1110501; Cat#A6964-100ML UltraPure0.5 M EDTA Invitrogen Cat#11568896 ROCK inhibitor Y-27632 Enzo Life Sciences Cat#ALX-270-333-M005 MycoZap Plus-CL Lonza Cat#VZA-2012 Penicillin/Streptomycin Gibco Cat#15140122 GlutaMAX Gibco Cat#35050061 L-glutamine Gibco Cat#25030081 FBS Gibco Cat#10270106 Matrigel, Growth Factor Reduced Corning Cat#356231 GeltrexReduced-Growth Factor BasementMembrane Matrix, LDEV-free, stem-cell qualified Gibco Cat#A1413302 Chemically Defined Lipid Concentrate Gibco Cat#11905031 Anti-Adherence Rinsing Solution STEMCELL Technologies Cat#07010 Purmorphamin Sigma-Aldrich; Miltenyi Biotec Cat#540220; Cat#130-104-465 CHIR 99021 Sigma-Aldrich Cat#SML1046 Dorsomorphin Sigma-Aldrich Cat#P5499 SB431542 Miltenyi Biotec Cat#130-105-336 GDNF R&D System Cat#212-GD-010 BDNF MACS Miltenyi Cat#130-096-811 EGF R&D System Cat#236-EG-200 FGF2 R&D System Cat#3718-FB-100 (Continued on next page) ll OPEN ACCESS e2 Cell 189, 1–24.e1–e24, March 19, 2026 Please cite this article in press as: Zink et al., Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy, Cell (2026), https://doi.org/10.1016/j.cell.2026.02.008 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP StemCell Technologies Cat#73886 Recombinant Human NT-3 Peprotech / Biozol Cat#450-03 FGF8-a R&D systems Cat#4745-F8-050 TGF-beta 3 STEMCELL Technologies Cat#78156 (+)-sodium L-ascorbate (Vitamin C) Sigma-Aldrich Cat#A4034 cis-4,7,10,13,16,19-Docosahexaenoic acid methyl ester (DHA) Sigma-Aldrich Cat#D2534 WNT antagonist IWR1 EMD Millipore Corp Cat#681669 Heparin Merck Cat#375095 ß-mercaptoethanol Gibco Cat#31350010 DMSO Sigma-Aldrich Cat#D2650-100ML SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat#34580 16% Paraformaldehyde (PFA) Thermo Fisher Scientific Cat#28906 Hoechst 33342 Invitrogen Cat#H3570 Donkey serum (DNS) Merck Millipore Cat#S30 Triton X-100 Sigma-Aldrich Cat#93443; Cat#93426 Tween 20 Sigma-Aldrich Cat#P1504 abberior STAR 635 (STAR RED) Abberior Cat#ST635-1002 DAPI Sigma-Aldrich Cat#D9542 MitoProbeTMRM Assay Kit for Flow Cytometry Invitrogen Cat#M20036 Tetramethylrhodamine methyl ester (TMRM) Invitrogen Cat#T668 Incucyte dye red Sartorius Cat#4717 Calcein AM Viability Dye Invitrogen Cat#C1430 MitoSpyOrange BioLegend Cat#424803 d 3 -Sildenafil CDN isotopes Cat#D-6366 HpaII NEB Cat#R0171 StuI NEB CatR0187 XbaI NEB Cat#R0145 FCCP Biozol Cat#SEL-S8276 Antimycin A Sigma-Aldrich Cat#A8674 Oligomycin Sigma-Aldrich Cat#O4876-5MG CCCP Sigma-Aldrich Cat#555602 Propidium iodide Invitrogen Cat#P1304MP Sildenafil citrate Sigma-Aldrich Cat#SML3033 KT5823 Biomol Cat#LKT-K7602.100 8-Br-cGMP Sigma-Aldrich Cat#203820 Sildenafil Selleckchem Cat#S468402 and S468403 Mowiol with 0.1% 1,4- Diazabicyclo[2.2.2]octan (DABCO) Carl Roth Cat#0713.1 LB Agar Sigma-Aldrich Cat#L2897 SuperFrost Plus glass slides VWR Cat#631-0447 Pro-Long Glass Antifade Mountant Invitrogen Cat#P36984 oligo d(T)18 primers Thermo Fisher Scientific Cat#SO132 dNTP mix Thermo Fisher Scientific Cat#10319879 Rnase OUT Recombinant Ribonuclease Inhibitor Invitrogen Cat#10777-019 M-MLV Reverse Transcriptase (200 U/μL) Invitrogen Cat#28025021 (Continued on next page) ll OPEN ACCESS Cell 189, 1–24.e1–e24, March 19, 2026 e3 Please cite this article in press as: Zink et al., Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy, Cell (2026), https://doi.org/10.1016/j.cell.2026.02.008 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Proteomics NPCs dataset This paper https://www.ebi.ac.uk/pride/archive/ projects/PXD059519; ProteomeXchange: PXD059519 Metabolomics NPCs dataset This paper https://massive.ucsd.edu/ProteoSAFe/ dataset.jsp?task= ee42368caf914a948db5983afe2460e4; MassIVE: MSV000097182 Experimental models: Cell lines Human iPSCs: HMGUi001-A Helmholtz Zentrum Mü nchen (HMGU) PMID: 29396371 Human iPSCs: HHUUKDi009-A Heinrich-Heine-Universitä t Dü sseldorf (HHUUKD) PMID: 28132834 Human iPSCs: CRMi003-A RUCDR Infinite Biologics PMID: 36459969 Human iPSCs: BIHi269-B Berlin Institute of Health (BIH) PMID: 36669241 Human iPSCs: HVRDi004-B Synthego PMID: 19911041 Human iPSCs: IUFi004-A Cell Applications PMID: 38217996 Human iPSCs: BIHi266-A Berlin Institute of Health (BIH) PMID: 36669241 Human iPSCs: WISCi004-B WiCell PMID: 18029452 Human iPSCs: UMGi014-C University Medical Center Goettingen (UMG) PMID: 33905594 Human iPSCs: HHUi001-A Universitä tsklinikum Dü sseldorf (HHU) PMID: 28132834 Human iPSCs: HHUi002-A Universitä tsklinikum Dü sseldorf (HHU) PMID: 28132834 Human iPSCs: HHUi003-C Universitä tsklinikum Dü sseldorf (HHU) PMID: 36137325 Human iPSCs: MDCi008-A Max Delbrü ck Center Berlin Buch (MDC) PMID: 35279592 Human iPSCs: MDCi009-A Max Delbrü ck Center Berlin Buch (MDC) PMID: 35279592 Human iPSCs: MDCi010-A Max Delbrü ck Center Berlin Buch (MDC) PMID: 35279592 Human iPSCs: BIHi267-B Berlin Institute of Health (BIH) PMID: 36669241 Human iPSCs: HMGUi001-A-64 Max Delbrü ck Center Berlin Buch (MDC) PMID: 33771987 Human iPSCs: HVRDi004-B-6 Max Delbrü ck Center Berlin Buch (MDC) PMID: 36741056 Human iPSCs: HVRDi004-B-7 Max Delbrü ck Center Berlin Buch (MDC) PMID: 36741056 Experimental models: Organisms/strains Germline Ndufs4 KO, C57/BL6/J background University of Washington PMID: 20534480 SURF1 KO pig, Large White background Avantea PMID: 29601977 Oligonucleotides See Table S5 for oligonucleotides N/A N/A Software and algorithms CellProfiler https://cellprofiler.org/ v.4.2.5.

    Flow Cytometry:

    Article Title: Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ACTN2 antibody (alpha-actinin), mouse monoclonal, clone EA-53 Sigma-Aldrich Cat#A7811; RRID: AB_476766 Anti-ATP5B antibody, rabbit polyclonal Sigma-Aldrich Cat#HPA001528; RRID: AB_1078242 NDUFA9 antibody, mouse monoclonal IgG, clone 20C11B11B11 Invitrogen Cat#459100; RRID: AB_10376187 Anti-SDHB, mouse monoclonal IgG2a, clone 21A11EA7 Abcam Cat#ab14714; RRID: AB_301432 Anti-UQCRSF1/RISP antibody, mouse monoclonal IgG2b, clone 5A5 Abcam Cat#ab14746; RRID: AB_301445 Doublecortin antibody (DCX), rabbit Cell Signaling Technology Cat#4604; RRID: AB_561007 Chemicals, peptides, and recombinant proteins StemMACSiPS-Brew XF, human Miltenyi Biotec Cat#130-104-368 mTeSRPlus STEMCELL Technologies Cat#100-0276 KnockOut-DMEM Gibco Cat#10829-018 KnockOutserum replacement (SR) Gibco Cat#10828-028 DMEM/F/12 Gibco Cat#31330038 DMEM glucose-free Gibco Cat#11966025 Glasgow-MEM Gibco Cat#11710035 Neurobasal A-Medium glucose-free Gibco Cat#A2477501 Neurobasal Gibco Cat#21103-049 Non-essential amino acids (MEM-NEAA) 100X Gibco Cat#11140-050 Sodium pyruvate Gibco Cat#11360070 B-27 with Vitamin A (50×) Gibco Cat#17504044 B-27 without Vitamin A (50×) Gibco Cat#12587010 N2 Supplement (100×) Gibco Cat#17502-048 DPBS, no calcium, no magnesium Gibco Cat#14190144 Accutase Thermo Fisher Scientific; Sigma-Aldrich Cat#A1110501; Cat#A6964-100ML UltraPure0.5 M EDTA Invitrogen Cat#11568896 ROCK inhibitor Y-27632 Enzo Life Sciences Cat#ALX-270-333-M005 MycoZap Plus-CL Lonza Cat#VZA-2012 Penicillin/Streptomycin Gibco Cat#15140122 GlutaMAX Gibco Cat#35050061 L-glutamine Gibco Cat#25030081 FBS Gibco Cat#10270106 Matrigel, Growth Factor Reduced Corning Cat#356231 GeltrexReduced-Growth Factor BasementMembrane Matrix, LDEV-free, stem-cell qualified Gibco Cat#A1413302 Chemically Defined Lipid Concentrate Gibco Cat#11905031 Anti-Adherence Rinsing Solution STEMCELL Technologies Cat#07010 Purmorphamin Sigma-Aldrich; Miltenyi Biotec Cat#540220; Cat#130-104-465 CHIR 99021 Sigma-Aldrich Cat#SML1046 Dorsomorphin Sigma-Aldrich Cat#P5499 SB431542 Miltenyi Biotec Cat#130-105-336 GDNF R&D System Cat#212-GD-010 BDNF MACS Miltenyi Cat#130-096-811 EGF R&D System Cat#236-EG-200 FGF2 R&D System Cat#3718-FB-100 (Continued on next page) ll OPEN ACCESS e2 Cell 189, 1–24.e1–e24, March 19, 2026 Please cite this article in press as: Zink et al., Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy, Cell (2026), https://doi.org/10.1016/j.cell.2026.02.008 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP StemCell Technologies Cat#73886 Recombinant Human NT-3 Peprotech / Biozol Cat#450-03 FGF8-a R&D systems Cat#4745-F8-050 TGF-beta 3 STEMCELL Technologies Cat#78156 (+)-sodium L-ascorbate (Vitamin C) Sigma-Aldrich Cat#A4034 cis-4,7,10,13,16,19-Docosahexaenoic acid methyl ester (DHA) Sigma-Aldrich Cat#D2534 WNT antagonist IWR1 EMD Millipore Corp Cat#681669 Heparin Merck Cat#375095 ß-mercaptoethanol Gibco Cat#31350010 DMSO Sigma-Aldrich Cat#D2650-100ML SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat#34580 16% Paraformaldehyde (PFA) Thermo Fisher Scientific Cat#28906 Hoechst 33342 Invitrogen Cat#H3570 Donkey serum (DNS) Merck Millipore Cat#S30 Triton X-100 Sigma-Aldrich Cat#93443; Cat#93426 Tween 20 Sigma-Aldrich Cat#P1504 abberior STAR 635 (STAR RED) Abberior Cat#ST635-1002 DAPI Sigma-Aldrich Cat#D9542 MitoProbeTMRM Assay Kit for Flow Cytometry Invitrogen Cat#M20036 Tetramethylrhodamine methyl ester (TMRM) Invitrogen Cat#T668 Incucyte dye red Sartorius Cat#4717 Calcein AM Viability Dye Invitrogen Cat#C1430 MitoSpyOrange BioLegend Cat#424803 d 3 -Sildenafil CDN isotopes Cat#D-6366 HpaII NEB Cat#R0171 StuI NEB CatR0187 XbaI NEB Cat#R0145 FCCP Biozol Cat#SEL-S8276 Antimycin A Sigma-Aldrich Cat#A8674 Oligomycin Sigma-Aldrich Cat#O4876-5MG CCCP Sigma-Aldrich Cat#555602 Propidium iodide Invitrogen Cat#P1304MP Sildenafil citrate Sigma-Aldrich Cat#SML3033 KT5823 Biomol Cat#LKT-K7602.100 8-Br-cGMP Sigma-Aldrich Cat#203820 Sildenafil Selleckchem Cat#S468402 and S468403 Mowiol with 0.1% 1,4- Diazabicyclo[2.2.2]octan (DABCO) Carl Roth Cat#0713.1 LB Agar Sigma-Aldrich Cat#L2897 SuperFrost Plus glass slides VWR Cat#631-0447 Pro-Long Glass Antifade Mountant Invitrogen Cat#P36984 oligo d(T)18 primers Thermo Fisher Scientific Cat#SO132 dNTP mix Thermo Fisher Scientific Cat#10319879 Rnase OUT Recombinant Ribonuclease Inhibitor Invitrogen Cat#10777-019 M-MLV Reverse Transcriptase (200 U/μL) Invitrogen Cat#28025021 (Continued on next page) ll OPEN ACCESS Cell 189, 1–24.e1–e24, March 19, 2026 e3 Please cite this article in press as: Zink et al., Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy, Cell (2026), https://doi.org/10.1016/j.cell.2026.02.008 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Proteomics NPCs dataset This paper https://www.ebi.ac.uk/pride/archive/ projects/PXD059519; ProteomeXchange: PXD059519 Metabolomics NPCs dataset This paper https://massive.ucsd.edu/ProteoSAFe/ dataset.jsp?task= ee42368caf914a948db5983afe2460e4; MassIVE: MSV000097182 Experimental models: Cell lines Human iPSCs: HMGUi001-A Helmholtz Zentrum Mü nchen (HMGU) PMID: 29396371 Human iPSCs: HHUUKDi009-A Heinrich-Heine-Universitä t Dü sseldorf (HHUUKD) PMID: 28132834 Human iPSCs: CRMi003-A RUCDR Infinite Biologics PMID: 36459969 Human iPSCs: BIHi269-B Berlin Institute of Health (BIH) PMID: 36669241 Human iPSCs: HVRDi004-B Synthego PMID: 19911041 Human iPSCs: IUFi004-A Cell Applications PMID: 38217996 Human iPSCs: BIHi266-A Berlin Institute of Health (BIH) PMID: 36669241 Human iPSCs: WISCi004-B WiCell PMID: 18029452 Human iPSCs: UMGi014-C University Medical Center Goettingen (UMG) PMID: 33905594 Human iPSCs: HHUi001-A Universitä tsklinikum Dü sseldorf (HHU) PMID: 28132834 Human iPSCs: HHUi002-A Universitä tsklinikum Dü sseldorf (HHU) PMID: 28132834 Human iPSCs: HHUi003-C Universitä tsklinikum Dü sseldorf (HHU) PMID: 36137325 Human iPSCs: MDCi008-A Max Delbrü ck Center Berlin Buch (MDC) PMID: 35279592 Human iPSCs: MDCi009-A Max Delbrü ck Center Berlin Buch (MDC) PMID: 35279592 Human iPSCs: MDCi010-A Max Delbrü ck Center Berlin Buch (MDC) PMID: 35279592 Human iPSCs: BIHi267-B Berlin Institute of Health (BIH) PMID: 36669241 Human iPSCs: HMGUi001-A-64 Max Delbrü ck Center Berlin Buch (MDC) PMID: 33771987 Human iPSCs: HVRDi004-B-6 Max Delbrü ck Center Berlin Buch (MDC) PMID: 36741056 Human iPSCs: HVRDi004-B-7 Max Delbrü ck Center Berlin Buch (MDC) PMID: 36741056 Experimental models: Organisms/strains Germline Ndufs4 KO, C57/BL6/J background University of Washington PMID: 20534480 SURF1 KO pig, Large White background Avantea PMID: 29601977 Oligonucleotides See Table S5 for oligonucleotides N/A N/A Software and algorithms CellProfiler https://cellprofiler.org/ v.4.2.5.

    Reverse Transcription:

    Article Title: Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-ACTN2 antibody (alpha-actinin), mouse monoclonal, clone EA-53 Sigma-Aldrich Cat#A7811; RRID: AB_476766 Anti-ATP5B antibody, rabbit polyclonal Sigma-Aldrich Cat#HPA001528; RRID: AB_1078242 NDUFA9 antibody, mouse monoclonal IgG, clone 20C11B11B11 Invitrogen Cat#459100; RRID: AB_10376187 Anti-SDHB, mouse monoclonal IgG2a, clone 21A11EA7 Abcam Cat#ab14714; RRID: AB_301432 Anti-UQCRSF1/RISP antibody, mouse monoclonal IgG2b, clone 5A5 Abcam Cat#ab14746; RRID: AB_301445 Doublecortin antibody (DCX), rabbit Cell Signaling Technology Cat#4604; RRID: AB_561007 Chemicals, peptides, and recombinant proteins StemMACSiPS-Brew XF, human Miltenyi Biotec Cat#130-104-368 mTeSRPlus STEMCELL Technologies Cat#100-0276 KnockOut-DMEM Gibco Cat#10829-018 KnockOutserum replacement (SR) Gibco Cat#10828-028 DMEM/F/12 Gibco Cat#31330038 DMEM glucose-free Gibco Cat#11966025 Glasgow-MEM Gibco Cat#11710035 Neurobasal A-Medium glucose-free Gibco Cat#A2477501 Neurobasal Gibco Cat#21103-049 Non-essential amino acids (MEM-NEAA) 100X Gibco Cat#11140-050 Sodium pyruvate Gibco Cat#11360070 B-27 with Vitamin A (50×) Gibco Cat#17504044 B-27 without Vitamin A (50×) Gibco Cat#12587010 N2 Supplement (100×) Gibco Cat#17502-048 DPBS, no calcium, no magnesium Gibco Cat#14190144 Accutase Thermo Fisher Scientific; Sigma-Aldrich Cat#A1110501; Cat#A6964-100ML UltraPure0.5 M EDTA Invitrogen Cat#11568896 ROCK inhibitor Y-27632 Enzo Life Sciences Cat#ALX-270-333-M005 MycoZap Plus-CL Lonza Cat#VZA-2012 Penicillin/Streptomycin Gibco Cat#15140122 GlutaMAX Gibco Cat#35050061 L-glutamine Gibco Cat#25030081 FBS Gibco Cat#10270106 Matrigel, Growth Factor Reduced Corning Cat#356231 GeltrexReduced-Growth Factor BasementMembrane Matrix, LDEV-free, stem-cell qualified Gibco Cat#A1413302 Chemically Defined Lipid Concentrate Gibco Cat#11905031 Anti-Adherence Rinsing Solution STEMCELL Technologies Cat#07010 Purmorphamin Sigma-Aldrich; Miltenyi Biotec Cat#540220; Cat#130-104-465 CHIR 99021 Sigma-Aldrich Cat#SML1046 Dorsomorphin Sigma-Aldrich Cat#P5499 SB431542 Miltenyi Biotec Cat#130-105-336 GDNF R&D System Cat#212-GD-010 BDNF MACS Miltenyi Cat#130-096-811 EGF R&D System Cat#236-EG-200 FGF2 R&D System Cat#3718-FB-100 (Continued on next page) ll OPEN ACCESS e2 Cell 189, 1–24.e1–e24, March 19, 2026 Please cite this article in press as: Zink et al., Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy, Cell (2026), https://doi.org/10.1016/j.cell.2026.02.008 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP StemCell Technologies Cat#73886 Recombinant Human NT-3 Peprotech / Biozol Cat#450-03 FGF8-a R&D systems Cat#4745-F8-050 TGF-beta 3 STEMCELL Technologies Cat#78156 (+)-sodium L-ascorbate (Vitamin C) Sigma-Aldrich Cat#A4034 cis-4,7,10,13,16,19-Docosahexaenoic acid methyl ester (DHA) Sigma-Aldrich Cat#D2534 WNT antagonist IWR1 EMD Millipore Corp Cat#681669 Heparin Merck Cat#375095 ß-mercaptoethanol Gibco Cat#31350010 DMSO Sigma-Aldrich Cat#D2650-100ML SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat#34580 16% Paraformaldehyde (PFA) Thermo Fisher Scientific Cat#28906 Hoechst 33342 Invitrogen Cat#H3570 Donkey serum (DNS) Merck Millipore Cat#S30 Triton X-100 Sigma-Aldrich Cat#93443; Cat#93426 Tween 20 Sigma-Aldrich Cat#P1504 abberior STAR 635 (STAR RED) Abberior Cat#ST635-1002 DAPI Sigma-Aldrich Cat#D9542 MitoProbeTMRM Assay Kit for Flow Cytometry Invitrogen Cat#M20036 Tetramethylrhodamine methyl ester (TMRM) Invitrogen Cat#T668 Incucyte dye red Sartorius Cat#4717 Calcein AM Viability Dye Invitrogen Cat#C1430 MitoSpyOrange BioLegend Cat#424803 d 3 -Sildenafil CDN isotopes Cat#D-6366 HpaII NEB Cat#R0171 StuI NEB CatR0187 XbaI NEB Cat#R0145 FCCP Biozol Cat#SEL-S8276 Antimycin A Sigma-Aldrich Cat#A8674 Oligomycin Sigma-Aldrich Cat#O4876-5MG CCCP Sigma-Aldrich Cat#555602 Propidium iodide Invitrogen Cat#P1304MP Sildenafil citrate Sigma-Aldrich Cat#SML3033 KT5823 Biomol Cat#LKT-K7602.100 8-Br-cGMP Sigma-Aldrich Cat#203820 Sildenafil Selleckchem Cat#S468402 and S468403 Mowiol with 0.1% 1,4- Diazabicyclo[2.2.2]octan (DABCO) Carl Roth Cat#0713.1 LB Agar Sigma-Aldrich Cat#L2897 SuperFrost Plus glass slides VWR Cat#631-0447 Pro-Long Glass Antifade Mountant Invitrogen Cat#P36984 oligo d(T)18 primers Thermo Fisher Scientific Cat#SO132 dNTP mix Thermo Fisher Scientific Cat#10319879 Rnase OUT Recombinant Ribonuclease Inhibitor Invitrogen Cat#10777-019 M-MLV Reverse Transcriptase (200 U/μL) Invitrogen Cat#28025021 (Continued on next page) ll OPEN ACCESS Cell 189, 1–24.e1–e24, March 19, 2026 e3 Please cite this article in press as: Zink et al., Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy, Cell (2026), https://doi.org/10.1016/j.cell.2026.02.008 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Proteomics NPCs dataset This paper https://www.ebi.ac.uk/pride/archive/ projects/PXD059519; ProteomeXchange: PXD059519 Metabolomics NPCs dataset This paper https://massive.ucsd.edu/ProteoSAFe/ dataset.jsp?task= ee42368caf914a948db5983afe2460e4; MassIVE: MSV000097182 Experimental models: Cell lines Human iPSCs: HMGUi001-A Helmholtz Zentrum Mü nchen (HMGU) PMID: 29396371 Human iPSCs: HHUUKDi009-A Heinrich-Heine-Universitä t Dü sseldorf (HHUUKD) PMID: 28132834 Human iPSCs: CRMi003-A RUCDR Infinite Biologics PMID: 36459969 Human iPSCs: BIHi269-B Berlin Institute of Health (BIH) PMID: 36669241 Human iPSCs: HVRDi004-B Synthego PMID: 19911041 Human iPSCs: IUFi004-A Cell Applications PMID: 38217996 Human iPSCs: BIHi266-A Berlin Institute of Health (BIH) PMID: 36669241 Human iPSCs: WISCi004-B WiCell PMID: 18029452 Human iPSCs: UMGi014-C University Medical Center Goettingen (UMG) PMID: 33905594 Human iPSCs: HHUi001-A Universitä tsklinikum Dü sseldorf (HHU) PMID: 28132834 Human iPSCs: HHUi002-A Universitä tsklinikum Dü sseldorf (HHU) PMID: 28132834 Human iPSCs: HHUi003-C Universitä tsklinikum Dü sseldorf (HHU) PMID: 36137325 Human iPSCs: MDCi008-A Max Delbrü ck Center Berlin Buch (MDC) PMID: 35279592 Human iPSCs: MDCi009-A Max Delbrü ck Center Berlin Buch (MDC) PMID: 35279592 Human iPSCs: MDCi010-A Max Delbrü ck Center Berlin Buch (MDC) PMID: 35279592 Human iPSCs: BIHi267-B Berlin Institute of Health (BIH) PMID: 36669241 Human iPSCs: HMGUi001-A-64 Max Delbrü ck Center Berlin Buch (MDC) PMID: 33771987 Human iPSCs: HVRDi004-B-6 Max Delbrü ck Center Berlin Buch (MDC) PMID: 36741056 Human iPSCs: HVRDi004-B-7 Max Delbrü ck Center Berlin Buch (MDC) PMID: 36741056 Experimental models: Organisms/strains Germline Ndufs4 KO, C57/BL6/J background University of Washington PMID: 20534480 SURF1 KO pig, Large White background Avantea PMID: 29601977 Oligonucleotides See Table S5 for oligonucleotides N/A N/A Software and algorithms CellProfiler https://cellprofiler.org/ v.4.2.5.



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